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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which results in chromosome instability, and therefore could add to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important part in advertising and marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two supplemental splice variants were documented but in-depth nucleotide sequence information and facts is not still accessible.
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide ordinarily has the epitope acknowledged with the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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